Publications

Background: Pericytes, a type of mural cells, exert important functions in the CNS. One major challenge in pericyte research is the lack of pericyte-specific and subpopulation-specific markers.

Methods: To address this knowledge gap, we first generated a novel transgenic mouse line in which vascular smooth muscle cells (vSMCs) are permanently labeled with tdTomato. Next, we isolated PDGFRβ⁺tdTomato^- pericytes and PDGFRβ⁺tdTomato⁺ vSMCs from the brains of these mice and subsequently performed RNAseq analysis to identify pericyte-enriched genes.

Results: Using this approach, we successfully identified 40 pericyte-enriched genes and 158 vSMC-enriched genes, which are involved in different biological processes and molecular functions. Using ISH/IHC analysis, we found that Pla1a and Cox4i2 were predominantly enriched in subpopulations of brain pericytes, although they also marked some non-vascular parenchymal cells.

Conclusions: These findings suggest that Pla1a and Cox4i2 preferably label subpopulations of pericytes in the brain compared to vSMCs, and thus, they may be useful in distinguishing these populations.

The article "The efficacy and safety of a single dose of Polyhexamethylene Biguanide gynaecologic solution versus a seven-dose regimen of vaginal clindamycin cream in patients with bacterial vaginosis" by M. Minozzi, S. Gerli, G.C. Di Renzo, E. Papaleo, A. Ferrari, published in Eur Rev Med Pharmacol Sci 2008; 12 (1): 59-65-PMID: 18401974 has been retracted by the Authors in accordance with the Publisher and the Editor in Chief. Following some concerns raised by a third party regarding a possible high degree of textual similarity with a previously published paper, the journal has started an investigation. The authors were informed of the ongoing investigation and were asked to provide original data and clarify several other issues. In response, the authors requested the retraction of the manuscript, citing discrepancies related to the article and expressing a lack of confidence in the accuracy of the information submitted. This article has been retracted. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/477.

The Editor in Chief and the Publisher are issuing an expression of concern regarding the following article: Unfer V, Carlomagno G, Rizzo P, Raffone E, Roseff S. Myo-inositol rather than D-chiro-inositol is able to improve oocyte and embryo quality in PCOS patients having a normal insulin response. Eur Rev Med Pharmacol Sci. 2011 Apr; 15(4): 452-457. PMID: 21608442. This Expression of Concern is issued following a notification from a third party concerning a potential conflict of interest involving Dr. Unfer's role at Loli Pharma, the company marketing inositol-based products. This expression of concern aims to notify a possible bias arising from an undeclared conflict of interest. The authors have been notified of both the ongoing investigation and the publication of this notice. https://www.europeanreview.org/article/929.

This comment raises concerns about the validity and reproducibility of a publication by Li et al. in Virus Genes (60:488-500, 2024. https://doi.org/10.1007/s11262-024-02096-1 ; PMID: 39103702), which aimed to investigate the role of aquaporin-3 in nasopharyngeal carcinoma (NPC) and its regulation by the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1). The central criticism focuses on using the CNE-1, CNE-2, and HONE-1 cell lines, which are recognized as unreliable for NPC research. There is an alarming prevalence of publications that continue to utilize these compromised cell lines, impeding genuine advancements in understanding NPC. Beyond the cell line authenticity issue, the study lacks relevant methodological information and justification for experimental design choices. We emphasize the need for raw data availability and comprehensive methodological information to ensure reproducibility. Ultimately, this comment calls for a more rigorous and multi-faceted approach to quality control in scientific publishing and embracing Open Science principles to ensure the reliability of published research.

Qianrong LI1, Jin CHEN1, Xiaoyin WANG1, Lin ZHUANG1, Zhi YU1, Dan YANG1 1Department of Obstetrics, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China Physiol Res 2025 Mar 24;74(1):93-104. doi: 10.33549/physiolres.935474. PMID: 40126146 The authors requested to correct the name of the Ethical Committee and the Acknowledgement. Gestational diabetes induction Animal protocol was approved by Ethical Review Committee of Sichuan Laboratory Animal Society SCAF20250425001. Healthy female SD rats (180-210 g) were housed under controlled condition as per guideline. Animals were feed with high fat diet for the duration of eight-week [18], Female rats were mated with male rats by keeping them in cage at 2:1 ratio and presence of sperm in vagina confirm by observing it in microscope, the day is counted as day 0 of protocol. Later STZ (25 mg/kg) was injected i.p. after 12 h of fasting [19], blood glucose was estimated for 72 h. Rats with 200 mg/dl of blood glucose considered as GD. Acknowledgements All the author of given review thankful to Hospital of Chengdu University of Traditional Chinese Medicine, China for providing facility to conduct presented work.

This corrects the article on p. e46 in vol. 40, PMID: 40259722.

[This corrects the article on p. 398 in vol. 16, PMID: 38425403.].

Matrix metalloproteinases are crucial proteolytic enzymes involved in the degradation and remodeling of the extracellular matrix within the ocular structures. Their expression and activity significantly influence aqueous humor dynamics and intraocular pressure in patients with glaucoma. Elevated intraocular pressure, a significant risk factor for glaucoma, is often associated with disturbed aqueous humor outflow. This review summarizes current findings on the role of matrix metalloproteinases in modulating aqueous humor and their potential as therapeutic targets in the treatment of glaucoma.

Although oligodendrocytes (OLs) synthesize laminin-γ1, the most widely used γ subunit, its functional significance in the CNS remains unknown. To answer this important question, we generated a conditional knockout mouse line with laminin-γ1 deficiency in OL lineage cells (γ1-OKO). γ1-OKO mice exhibit weakness/paralysis and die by post-natal day 33. Additionally, they develop blood-brain barrier (BBB) disruption in the cortex and striatum. Subsequent studies reveal decreased major facilitator superfamily domain containing 2a expression and increased endothelial caveolae vesicles, but unaltered tight junction protein expression and tight junction ultrastructure, indicating a transcellular, rather than a paracellular, mechanism of BBB breakdown. Furthermore, significantly reduced OL lineage cells, OL precursor cells (OPCs), proliferating OPCs, and mature OLs are observed in γ1-OKO brains in a region-specific manner. Consistent with this finding, various defects in myelination are detected in γ1-OKO brains at biochemical and ultrastructural levels. Overall, these results highlight important roles of OL-derived laminin-γ1 in BBB maintenance and OL biology (proliferation, differentiation, and myelination).